We have many collaborators, some of our active collaborators are listed here (apologies to those we have forgotten!)
Mathias is helping us to use and understand gold nanoparticles
Bernard is involved in mathematical understanding of both self assembled bionano structures and self-replicators.
Anne helps us to understand the mathematical feasibility of self-replicators.
We work with Gary on understanding phage proteins, especially those involved in interaction with DNA.
We have a long-standing collaboration with Kenji who provides us with his expertise in cryo-EM to understand 3D structures of some of our favourite proteins.
Hideki is a cryo-EM expert who is helping us to gain high-resolution insights into some of our designed proteins.
We work with Julian on DNA nanotechnology projects and his expertise in producing DNA aptamers is especially useful.
We are working together with Ilia to try to combine our bionano with his expertise with lipids.
Craig helps us to understand and visualise unusual geometries.
Elżbieta is our partner in "Symfonia" funded research and brings expertise in TEM.
- The Glatt lab - our partners in crime
- Our institute - The Malopolska Centre of Biotechnology
Positions AvailableWe will soon be announcing positions as follows:
Two PhD studentship to research unsual gyrases funded by OPUS
One postdoctoral fellow to research unusual gyrases funded by OPUS
Professor Heddle is undoubtedly the greatest genius of our ageJonathan Heddle
I thought I was applying to a different labSoumyananda Chakraborti
Joining the lab is simply a small part of a much larger plan to take over the worldDmitry Ghilarov
I went to sleep in Tokyo and woke up in Poland. I think I may have been kidnappedYusuke Sakai
FundingWe are grateful to the various bodies who fund our research:
I am originally from India, working as postdoctoral fellow in MCB. In Heddle lab I am working with different protein cages their Biophysical characterization and their cellular delivery. I am experienced researcher; before joining MCB, Poland I worked in Indiana University, USA and Institute Curie, Paris. My two major areas of expertise are synthetic and chemical biology. I did my PhD from Calcutta University, India in the year 2013 in the field of Biophysics. Other than science I am interested in travelling and reading.
Biochemist from Moscow, Russia. After several years of postdoctoral studies in Russia and UK I joined the Heddle lab as an independent fellow. I hope that together with Jonathan we can find the answer to one of the scientific questions that bothered me for a long time - how Qnr proteins protect gyrase against fluoroquinolones. I'm passionate about science and ballroom dancing and enjoy travelling, tasting good food, studying arts and history. You can access my POLONEZ project page here
I’m a DNA nanotechnology researcher in Heddle lab. I have researched about DNA origami nanomechanical device in Japan before and I’m currently dedicating myself to developing innovative nano-scale devices made of DNA. I’m also attracted to and investigated RNA biochemistry and gene expression system, a sophisticated decoding machinery equipped in all organisms.
TRAP is a naturally occurring protein which role is to control the tryptophan synthesis in some bacterial species. This 11-sided protein, after incorporation of specific mutations and its incubation with nanogold particles, was found out to form special cage-like structures, which could have some interesting medical applications and could give us new insight into the rules of protein geometry. I take part in the research which aims to understand the formation of these structures but most of all I am involved in the production of novel proteins and testing the assembled ones for their interactions with various eukaryotic cell lines. My findings may help to design a new system for the safe cargo delivery, like drug molecules, to the inside of cells.
DNA gyrase is an important antibiotic target and understanding and developing new inhibitors against it will be a useful weapon in the fight against antibacterial resistance, which is a growing threat to human survival. Topoisomerase-targeting pentapeptide repeat proteins (TTPRPs) may help us achieve this goal. My goal is to understand PRP action using structural biology methods. It will be helpful in the fight against antibacterial resistance.I am also interested in discovering other anti-microbials like bacteriocins. My other scientific interest is recombinant proteins production (especially hard cases).
Put together gold nanoparticles and mutated TRAP protein (containing cysteine) and what will you get? Intriguing nanostructures - stable and with unique geometrical arrangements. Recent work in the Heddle Laboratory has suggested that certain gold nanoparticles can interact with proteins containing cysteine residues to promote protein-protein bonds, which can result in production of highly stable artificial protein cages. The recipe sounds simple but chemistry behind it is quite complex. The role of gold nanoparticles in forming these protein cages is crucial but not really understood. In this project, I focus on finding what kind of gold nanoparticles (in terms of size, shape and surface chemistry) and reaction conditions can promote cage formation. Establishing the mechanism of this process would be a great step towards developing new ways of assembling proteins into interesting nanostructures.
I'm a PhD math student at the Jagiellonian University always ready to broaden my horizons in different fields. My goal in Heddle's Lab is to understand the geometry of protein cages. Professionally - particularly interested in the rigidity theory and mathematical aspects of origami. Privately incurable dance lover (especially cuban salsa), mountain hiker and yerba mate fan.
My research interests include cell biology, cancer biology and immunology. During PhD studies my research focused on molecular mechanisms of activation receptors of immune cells and participation of proteins, like tyrosine kinases, in activation of cells with microbial components. In my current research I am using of various approaches, based on both biochemistry and bioinformatics, to study the structure of TRAP protein. My interest focuses on the use of mutated TRAP proteins, especially their capacity for forming a cage-like structures in the presence of gold nanoparticles, as potential nanodevices for cell-targeting. To pursue this goal I’m going to conduct tests on different cell lines using wide arrange of biochemical and imaging techniques.
After several years came back to academia from industry - technology transfer - you're doing it wrong! ;) But being more serious. I am a structural biologist with an expertise in X-ray macromolecular crystallography. For the past several years I've been working in private sector of science, purifying, crystallizing and solving 3D structures of proteins and protein complexes. Recently I've decided to broaden my horizons as a structural biologist, and start to investigate structures of protein complexes by using cryo-EM..
I received my Ph.D. in Chemistry from University of Gdansk (Poland) and carried out my postdoctoral research at National Cancer Institute, NIH, Bethesda, USA. My research focuses on peptide and protein design as an approach to understanding macromolecule structure and function. I have particular interest in peptide design and synthesis, enzymology and developing new enzyme inhibitors. In the Heddle Lab I carried out a structure–activity relationship (S.A.R.) study of the TRAP protein sequence to define the importance of each residue and the effect of a single substitution on the self assembly properties.
After working for a brief time in industry where I was involved in topoisomerase production and testing I moved to the Heddle lab where I am helping to investigate how Qnr and related proteins interact with DNA gyrase.
I am a PhD Student in the field: biological sciences, the discipline of biology, Jagiellonian University, Institute of Zoology, specialization: genetics and biology of reproduction. The basic aim of the Ph.D studies in my department is to examine the distribution, ultrastructure, mode of transovarial transmission and systematic affinity of symbionts in bodies of selected insects, phylogenetic relations between them and their host (Preludium 9, NCN grant for the young researchers). In the Heddle lab I work as a technician on the Symfonia project as a specialist of protein electron microscopy. Main tasks: preparation protein samples for TEM (Jeol JEM 2100), Cryo-electron microscopy (Cryo-EM); handling and using Vitrobot, TEM/Cryo-EM; data collection and analysis from TEM/Cryo-EM. More information about scientific achievements here.
Originally coming from Germany, I joined the Heddle lab as a PhD student. During my PhD project I am working on stabilisation hydrophobic proteins using DNA origami based devices. Before I started this project, I studied Biology in Hamburg, Germany. During my master at the university hospital of Eppendorf I did research on the structure of biofilm inducing proteins.
Origami refers to the art of folding and sculpting a flat sheet of paper into arbitrarily shaped objects and you can do almost the same with DNA. DNA origami allows us to easily design and build arbitrary structures using DNA strands as building block. In Heddle Lab I work as technician on the POLONEZ3 project focused on producing a programmable DNA nanorobots with an ability to act as drug carry capsules. It is still a new and an exciting field which may be fruitful in developing novel drug delivery systems. Before joining Heddle's Lab I was working as QC scientist in pharmaceutical industry.
I am a Bioinformatician from India and have joined Heddle's lab as a PhD student and here I am trying to develop a DNA nanodevice which captures for interaction with artificial cells. Before joining Heddle's lab, I was working in Indian Institute of Science as a Research Assistant and completed couple of projects. Outside of the lab, I am interested in Photography and travelling
I am currently a Masters student at Jagiellonian University in Bioinformatics and Applied Biophysics, after obtaining a BSc in Biochemistry. My primary scientific interests are Synthetic Biology and general Drug Development, along with novel Drug Delivery systems. Here, at Heddle Lab, II am involved in a POLONEZ3 research project, which aims to develop a system of drug delivery based on a programmable DNA robot. I am trying to focus on Biophysical approach to this subject. During my previous studies, I have researched the pathogenesis of neuropsychiatric diseases
DNA gyrase is one of the most important targets for antibacterial drugs. However, recently it was found in single cell eukaryotic parasites such as Plasmodium which causes malaria and Toxoplasma which causes toxoplasmosis. Better understanding their structure and mechanism of working could lead to future drug discovery. In my project, I will focus on characterisation of Toxoplasma gyrase – both biochemically and structurally.
My education is related to fields of chemistry and biology and focuses on biochemistry, primarily on protein biochemistry. My research topics include protein – ligand and protein-protein interactions, biochemical reaction kinetics which includes enzyme kinetics and kinetics of other reactions. I have worked on energetics of biochemical reactions and systems using calorimetric techniques. I have worked on protein stability and structure in solution which includes chemical and thermal denaturation processes. In my research I have used HPLC techniques for analytical and semi-preparation scale with respect to peptides, alkaloids, polyphenols, steroids and sugars and others.
Currently I am a Biochemistry Master student on the Jagiellonian Univeristy. During my Bachelor studies I was working with cancer cells and lentiviral vectors but I decided to change completely field of my interesting. I have joined Heddle Lab and I will be doing research on TRAP protein. I want to make TRAP cages but instead of gold nanoparticles I will be using a new approach. This project is something totally new for me so I am very curious about my future results
I am a master student at molecular biotechnology at Jagiellonian University, my former experience during my bachelor studies focused mainly in bacterial genetics. As a part of DNA origami group in Heddle Lab we are aiming to develop molecular systems for manipulation of proteins for use in artificial cells. For this purpose we will use (yes, you guessed well) DNA origami! Personally, I am also interested in cinematography, languages and travelling.